HiLoad Superdex™ 200 pg preparative SEC columns > 실험용 소모품

High mechanical strength and hydrophilicity make this dextran-agarose composite resin suitable for larger scale applications that require high flow rates, minimal nonspecific interactions, and fast, effective cleaning-in-place (CIP) protocols.

Successful use of Superdex 200 pg columns in research and process development

Superdex 200 prep grade columns are an excellent choice for many applications, including:

• automated monoclonal antibody production
• separation of serum proteins
• purification of human papillomavirus 16 L2E7 fusion protein

How to scale up column chromatography to process larger sample volumes

When scaling up size exclusion chromatography columns in order to process larger sample volumes in a single step, consider the following steps:

1. Optimize high-resolution separation at small scale.
2. Maintain the sample-to-column volume ratio and the sample concentration.
3. Increase the column volume by increasing the cross-sectional area (inner diameter) of the column.
4. Maintain the bed height.
5. Run the separation at the same linear flow rate as used on the smaller column.